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Ralstonia solanacaerum is one of the most devastating bacteria causing bacterial wilt disease in more than 200 species of plants, especially family Solanaceae. To cope with this, plants evolved different resistance mechanisms depending on the signal transduction after the perception. Phosphorylation is the central regulation of the signal transduction pathway. As the earliest signaling events are activated within minutes, we investigated comparative phosphoproteomics analysis of the stems of resistant and susceptible tomatoes at 15 min, 30 min, 24 h, and 48 h after inoculation by Ralstonia solanacearum to find out the phosphorylated proteins involved in the induced resistance.