Neurokinin-A (NKA) is only active when its C-terminus is amidated. B2(OH2) is thought to interact with Dha and cleave the peptide, leaving the amide on the C-terminus of the left-hand peptide. PeptideID MS/MS experiment was performed in e.coli lysate with overevpressed recombinant NKA-eH3 to prove diBoron activity in biologically relevant context. Overall analysis of the Dha generation and consumption on e.coli lysate was also performed.