To characterize the cellular targets of the ASFV pI73R, we carried out an Immunoprecipitation Mass-Spectrometry (IP-MS) experiment. Whole-cell extracts prepared from PAM cells which infected with ASFV I73R-mHA were used to immunoprecipitate endogenous targets pI73R, with a matched IgG serving as a control. The immunoprecipitated proteins were then subjected to LC-MS/MS.