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PXD029665 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReprocessed dataset with Skyline Batch: SWATH Analysis of Yeast Proteome over Time in Response to Osmotic Stress
DescriptionSWATH analysis of Yeast Proteome over time in response to Osmotic Stress. We sampled cell cultures in biological triplicates at six time points following the application of osmotic stress and acquired single injection DIA datasets on a high-resolution 5600 tripleTOF instrument operated in SWATH mode. Proteins were quantified by the targeted extraction and signal integration from the SWATH-MS datasets of peak groups representing proteotypic peptides for specific yeast proteins. REPROCESSING METHODS: Skyline Batch is a newly developed Windows forms application that enables easy and consistent reprocessing of data with Skyline. Skyline has made previous advances in this direction; however, none enable seamless automated reprocessing of local and remote files. Skyline keeps a log of all the steps that were taken in the document; however, reproducing these steps takes time and allows room for human error. Skyline also has a command-line interface, enabling it to be run from a batch script, but using the program in this way requires expertise in editing these scripts. By formalizing the workflow of a highly used set of batch scripts into an intuitive and powerful user interface, Skyline Batch can reprocess data stored in remote repositories just by opening and running a Skyline Batch configuration file. When run, a Skyline Batch configuration downloads all necessary remote files and then runs a four-step Skyline workflow. By condensing the steps needed to reprocess the data into one file, Skyline Batch gives researchers the opportunity to publish their processing along with their data and other analysis files. These easily run configuration files will greatly increase the transparency and reproducibility of published work. Skyline Batch is freely available at https://skyline.ms/batch.url.
ReviewLevelPeer-reviewed dataset
DatasetOriginReanalysis of PXD001010
RepositorySupportSupported dataset by repository
PrimarySubmitterBrendan MacLean
SpeciesList scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
ModificationListCarbamidomethyl; Oxidation
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-10 20:57:51ID requested
12021-11-12 15:08:05announced
22022-01-07 13:21:11announced: Added publication
Publication List
Marsh AN, Sharma V, Mani SK, Vitek O, MacCoss MJ, MacLean BX, Skyline Batch: An Intuitive User Interface for Batch Processing with Skyline. J Proteome Res, 21(1):289-294(2022) [pubmed]
Keyword List
submitter keyword: Skyline, workflow, reproducible, data analysis, reprocessing, software, mass spectrometry
Contact List
Michael MacCoss
contact affiliationDepartment of Genome Sciences, University of Washington, Seattle, WA, USA
contact emailmaccoss@uw.edu
lab head
Brendan MacLean
contact affiliationDepartment of Genome Sciences, University of Washington, Seattle, WA, USA
contact emailbrendanx@uw.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI