CCDC12 is a newly discovered potential oncogene by our team, and it is proposed to observe the molecules that have interaction with CCDC12 by the technology of proteomic sequencing to map the interaction network of CCDC12. The refined molecular interaction network will help to better observe the role of CCDC12 in cancer for targeted modulation. In this study, we used 4plex iTRAQ technology to sequence proteins from colon cancer cells overexpressing CCDC12, and finally found the differential proteins.