Update publication information. In this study, we investigated the secretion of OMVs from a clinical H. parasuis isolate strain (H45). In addition, we further analyzed the characterization, the comprehensive proteome and the virulence potential of OMVs. Our data demonstrated that H. parasuis could secrete OMVs into the extracellular milieu during infection. Using LC-MS/MS identification and bio-information analysis, we identified 588 different proteins associated with OMVs. And we also analyzed the subcellular location and biological function of those proteins. These proteins are mainly involved in immune and iron metabolism.