PXD029340 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Insight into the interplay between thiol oxidoreductases of oxidative activity of Campylobacter jejuni |
Description | The bacterial proteins of the Dsb family catalyze the formation of disulfide bridges between cysteine residues that stabilize protein structures and ensure their proper functioning. Here we report the detailed analysis of the Dsb pathway of Campylobacter jejuni. The oxidizing Dsb system of this pathogen is unique because it consists of two monomeric DsbAs (DsbA1 and DsbA2) and one dimeric bifunctional protein (C8J_1298). Previously, we showed that DsbA1 and C8J_1298 are redundant. Here we unraveled the interaction between the two monomeric DsbAs by in vitro and in vivo experiments and by solving their structures and found that both monomeric DsbAs are dispensable proteins. Their structures confirmed that they are homologs of EcDsbL. The slight differences seen in the surface charge of the proteins do not affect the interaction with their redox partner. Comparative proteomics showed that several respiratory proteins, as well as periplasmic transport proteins, are targets of the Dsb system. Some of these, both donors and electron acceptors, are essential elements of C. jejuni respiratory process under oxygen-limiting conditions in the host intestine. The data presented provides detailed information on function of the C. jejuni Dsb system, identifying it as a potential target for novel antibacterial molecules. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-22 |
AnnouncementXML | Submission_2022-02-22_04:23:24.766.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD029340 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Agata Malinowska |
SpeciesList | scientific name: Campylobacter jejuni subsp. jejuni 81116; NCBI TaxID: 407148; |
ModificationList | methylthiolated residue; monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-10-25 06:44:31 | ID requested | |
⏵ 1 | 2022-02-22 04:23:26 | announced | |
Publication List
Bana, ś AM, Bocian-Ostrzycka KM, Dunin-Horkawicz S, Ludwiczak J, Wilk P, Orlikowska M, Wyszy, ń, ska A, D, ą, browska M, Plichta M, Spodzieja M, Pola, ń, ska MA, Malinowska A, Jagusztyn-Krynicka EK, and Their Impact on Bacterial Physiology and Pathogenesis. Int J Mol Sci, 22(24):(2021) [pubmed] |
Keyword List
submitter keyword: Dsb, Campylobacter, bacteria |
Contact List
Katarzyna Jagusztyn-Krynicka |
contact affiliation | Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Poland |
contact email | kjkryn@gmail.com |
lab head | |
Agata Malinowska |
contact affiliation | IBB PAS |
contact email | esme@ibb.waw.pl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029340
- Label: PRIDE project
- Name: Insight into the interplay between thiol oxidoreductases of oxidative activity of Campylobacter jejuni