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PXD028943 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomics of TrkA and TrkB signaling in SH-SY5Y neuroblastoma cells
DescriptionHuman neuroblastoma cells (SH-SY5Y) were engineered to have stable expression of either TrkA (NTRK1) or TrkB (NTRK2) receptors. The cells were pretreated with 0.5 μM Trametinib, 2.5 μM Gefitinib, and combination of 0.25 μM Trametinib and 1.25 μM Gefitinib. Stimulation of TrkA and TrkB-expressing SH-SY5Y cells was carried out 30 minutes following inhibitor addition, using 100 ng/mL of recombinant NGF (#450-01) and BDNF (#450-02), respectively (both from Peprotech). The stimulated and unstimulated controls are included.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKieran Wynne
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-05 02:00:24ID requested
12022-04-13 03:46:36announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Cancer (B/D-HPP), Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project
submitter keyword: Phosphoproteomics, Neuroblastoma.
Contact List
Boris Kholodenko
contact affiliationSystems Biology Ireland, University College Dublin, Belfield, Dublin 4.
contact emailBoris.kholodenko@ucd.ie
lab head
Kieran Wynne
contact affiliationUniversity College Dublin
contact emailkieran.wynne1@ucd.ie
dataset submitter
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Dataset FTP location
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