PXD028627

PXD028627 is an original dataset announced via ProteomeXchange.

Title	Next-generation Serology by Mass Spectrometry:Readout of the SARS-CoV-2 Antibody Repertoire
Description	Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS, a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses new advances in protein mass spectrometry (MS) for multi-parametric readout of antibodies, with new metrics like Ion Titer (IT) and degree of clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells. We apply Ig-MS to plasma from subjects with severe & mild COVID-19, using the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 as the bait for antibody capture. Importantly, we report a new data type for human serology, with compatibility to any recombinant antigen to gauge our immune responses to vaccination, pathogens, or autoimmune disorders.
HostingRepository	PanoramaPublic
AnnounceDate	2022-09-24
AnnouncementXML	Submission_2022-09-24_16:02:15.036.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Young Ah Goo
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	dHex; Hex(3)HexNAc(4); NeuAc; Hex(4)HexNAc(4); Hex(5)HexNAc(4); HexNAc; HexNAc(1)dHex(1)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-18 14:30:01	ID requested
⏵ 1	2022-09-24 16:02:15	announced

Melani RD, Des Soye BJ, Kafader JO, Forte E, Hollas M, Blagojevic V, Negr, ã, o F, McGee JP, Drown B, Lloyd-Jones C, Seckler HS, Camarillo JM, Compton PD, LeDuc RD, Early B, Fellers RT, Cho BK, Mattamana BB, Goo YA, Thomas PM, Ash MK, Bhimalli PP, Al-Harthi L, Sha BE, Schneider JR, Kelleher NL, Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire. J Proteome Res, 21(1):274-288(2022) [pubmed]

submitter keyword: Glycan, Full MS scan

Young Ah Goo
contact affiliation	Northwestern University
contact email	young.goo@northwestern.edu
lab head
Young Ah Goo
contact affiliation	Northwestern University
contact email	young.goo@northwestern.edu
dataset submitter

Panorama Public dataset URI