PXD028503

PXD028503 is an original dataset announced via ProteomeXchange.

Title	Identification of the Polycomb repressive complex 2 and its interacting proteins in Paramecium tetraurelia
Description	Please provide an overall description of your study, think something similar in scope to the manuscript abstractn Paramecium tetraurelia, Ezl1 (Homolog of EZH2) and PtCAF1 (homolog of RbAp46/48) play important roles in genome rearrangement by disrupting the transposon and transposon-derived DNA elimination. Their influences on are very similar, which make us speculate they belong to the same complex. After verifying their interaction, we speculate the complex is polycomb repressive complex 2 (PRC2) and want to find the other components of this complex. We first tagged PtCAF1 with FlagHA and used anti-HA antibody to enrich for the interacting proteins, coupled with mass spectrometry analyses (previous submission). In this way, we identified the core components of PRC2, as well as some interacted proteins with this complex. In a follow up dataset, we tagged two other subunits of the PRC2 complex we identified, Ezl1 and Eed, with FlagHA,used an anti-HA antibody to enrich for the interacting proteins, coupled with mass spectrometry analyses, and verified the complex.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:47:04.140.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manfred Heller
SpeciesList	scientific name: Paramecium tetraurelia; NCBI TaxID: 5888;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-09-16 02:15:32	ID requested
1	2023-05-10 13:48:08	announced
⏵ 2	2023-11-14 08:47:05	announced	2023-11-14: Updated project metadata.

Wang C, Solberg T, Maurer-Alcal, á XX, Swart EC, Gao F, Nowacki M, A small RNA-guided PRC2 complex eliminates DNA as an extreme form of transposon silencing. Cell Rep, 40(8):111263(2022) [pubmed]

submitter keyword: Polycomb repressive complex 2,Paramecium tetraurelia, Immunoprecipitation

Mariusz Nowacki
contact affiliation	Institute of Cell Biology, University of Bern
contact email	mariusz.nowacki@unibe.ch
lab head
Manfred Heller
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland
contact email	pmscf@dbmr.unibe.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD028503

PRIDE project URI

• PRIDE
  1. PXD028503
     1. Label: PRIDE project
     2. Name: Identification of the Polycomb repressive complex 2 and its interacting proteins in Paramecium tetraurelia