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The excretory secretory products and positive sera of Clonorchis sinensis were collected for identifying the proteins presented in different infection periods, which specific to the sera of C. sinensis, but not combined with Fasciola hepatica, and Schistosoma japonicum. The White Japanese rabbits were artificially infected with C. sinensis, and the sera was collected at 7 days post-infection (dpi), 14 dpi, 35 dpi and 77 dpi. To identify the specific proteins to C. sinensis, Co-Immunoprecipitation (Co-IP) assay was used to pull down the sera root in C. sinensis, F. hepatica, and S. japonicum respectively by shotgun liquid chromatography tandem-mass spectrometry (LC-MS/MS).