Update publication information. We constructed non-flocculating strain PLY01 from industrial flocculating strain SPSC01. In the presence of 5.0 g/L acetic aicd stress, SPSC01 showed better growth and fermentation performance compared to PLY01. For probing mechanism of improved acetic acid tolerance induced by flocculation, we performed phosphoproteomic analysis to mine key proteins whose phosphorylation level has changed and regulation network.