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PXD028052 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA hierarchical transcriptional network controls appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae
DescriptionRice blast is a pervasive and devastating disease that threatens rice production across the world. In spite of its importance to global food security, however, the underlying biology of plant infection by the blast fungus Magnaporthe oryzae remains poorly understood. In particular, it is unclear how the fungus elaborates a specialised infection cell, the appressorium, in response to surface signals from the rice leaf. Here, we report the identification of a network of temporally co-regulated transcription factors that act downstream of the Pmk1 mitogen-activated protein kinase pathway to regulate gene expression during appressorium-mediated plant infection. We show that this tiered regulatory mechanism involves Pmk1-dependent phosphorylation of the Hox7 homeobox transcription factor which regulates genes associated with induction of major physiological changes required for appressorium development, including cell cycle control, autophagic cell death, turgor generation and melanin biosynthesis, as well as controlling a further set of virulence-associated transcription factor-encoding genes. Pmk1-dependent phosphorylation of Mst12 then regulates gene functions involved in septin-dependent cytoskeletal re-organisation, polarised exocytosis and effector gene expression necessary for plant tissue invasion.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterFrank Menke
SpeciesList scientific name: Pyricularia oryzae; NCBI TaxID: 318829;
ModificationListOxidation; Phospho
InstrumentOrbitrap Eclipse; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-20 07:55:27ID requested
12022-09-05 14:52:49announced
Publication List
Os, é, s-Ruiz M, Cruz-Mireles N, Martin-Urdiroz M, Soanes DM, Eseola AB, Tang B, Derbyshire P, Nielsen M, Cheema J, Were V, Eisermann I, Kershaw MJ, Yan X, Valdovinos-Ponce G, Molinari C, Littlejohn GR, Valent B, Menke FLH, Talbot NJ, Appressorium-mediated plant infection by Magnaporthe oryzae is regulated by a Pmk1-dependent hierarchical transcriptional network. Nat Microbiol, 6(11):1383-1397(2021) [pubmed]
Keyword List
submitter keyword: Magnaporthe oryzae, appressorium, Hox7, transcriptional profiling, Pmk1, MAP kinase, phosphorylation
Contact List
Frank Menke
contact affiliationThe Sainsbury Laboratory
contact emailfrank.menke@tsl.ac.uk
lab head
Frank Menke
contact affiliationThe Sainsbury Laboratory
contact emailfrank.menke@tsl.ac.uk
dataset submitter
Full Dataset Link List
Panorama Public dataset URI