In order to analyse the tissue enriched expression of proteins using Selected reaction monitoring approach, a set of 45 proteins were selected. Targeted proteomics data for the selected proteins was acquired for nine different tissues in Labeo rohita including brain, embryo, eye, female gonad, heart, kidney, liver, male gonad and spinal cord. Comparative analysis of monitored peptides and proteins showed their tissue specific/enriched expression in a particular type of sample.