PXD027988

PXD027988 is an original dataset announced via ProteomeXchange.

Title	Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation
Description	An aberrant immune response developed early in life may trigger inflammatory bowel disease (IBD) and food allergies (e.g., celiac disease). Fecal levels of immune markers categorize an inflammatory response (e.g., food allergy, autoimmune) paralleled with the initial microbial colonization. The immunoaffinity assays are routinely applied to quantify circulating immune protein markers in blood/serum. However, a reliable, multiplex assay to quantify fecal levels of immune proteins is unavailable. We developed mass spectrometry assays to simultaneously quantify fecal calprotectin, myeloperoxidase, eosinophil-derived neurotoxin, eosinophil cationic protein, alpha 1 antitrypsin 1, and adaptive immunity effectors in 134 neonatal stool swabs. We optimized extraction and proteolytic protocol and validated the multiplex assay in terms of linearity of response (>100; typically 0.04 to 14.77 µg/mg of total protein), coefficient of determination (R2; >0.99), the limit of detection (LOD; 0.003 to 0.04 µg/mg of total protein), the limit of quantification (LOQ; 0.009 to 0.122 µg/mg of total protein) and robustness. The median CV of intra- and interday precision was 9.8% and 14.1%, respectively. We quantified breast milk-derived IGHA2 to differentiate meconium from feces samples and to detect the first food intake. An early life profiling of immune markers reflects disrupted intestinal homeostasis, and it is perhaps suitable for pre-symptomatic interception of IBD and food allergies.
HostingRepository	PanoramaPublic
AnnounceDate	2021-08-17
AnnouncementXML	Submission_2021-08-17_20:26:42.019.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Zdenek Spacil
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	6495B Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-17 20:18:41	ID requested
⏵ 1	2021-08-17 20:26:42	announced

Vidova V, Benesova E, Klanova J, Thon V, Spacil Z, Simultaneous quantitative profiling of clinically relevant immune markers in neonatal stool swabs to reveal inflammation. Sci Rep, 11(1):10222(2021) [pubmed]

submitter keyword: fecal inflammatory markers, microbial colonization, immune response, targeted quantitative proteomics, mass spectrometry

Zdenek Spacil
contact affiliation	Masaryk University, Faculty of Science, RECETOX
contact email	spacil@recetox.muni.cz
lab head
Zdenek Spacil
contact affiliation	Masaryk University, Faculty of Science, RECETOX
contact email	spacil@recetox.muni.cz
dataset submitter

Panorama Public dataset URI