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PXD027835 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleGrowth Rate Dependent and Nutrient-specific Gene Expression Resource Allocation in Fission Yeast
DescriptionCellular resources are limited and their relative allocation to gene expression programmes determines physiological states and global properties such as the growth rate. Quantitative studies using various growth conditions have singled out growth rate as a major physiological variable explaining relative protein abundances. Here, we used the simple eukaryote Schizosaccharomyces pombe to determine the importance of growth rate in explaining relative changes in protein and mRNA levels during growth on a series of non-limiting nitrogen sources. Although half of fission yeast genes were significantly correlated with the growth rate, this came alongside widespread nutrient-specific regulation. Proteome and transcriptome often showed coordinated regulation but with notable exceptions, such as metabolic enzymes. Genes positively correlated with growth rate participated in every level of protein production with the notable exception of RNA polymerase II, whereas those negatively correlated mainly belonged to the environmental stress response programme. Critically, metabolic enzymes, which represent ~55-70% of the proteome by mass, showed mainly condition-specific regulation. Specifically, many enzymes involved in glycolysis and NAD-dependent metabolism as well as the fermentative and respiratory pathways were condition-dependent and not consistently correlated with growth. In summary, we provide a rich account of resource allocation to gene expression in a simple eukaryote, advancing our basic understanding of the interplay between growth-rate dependent and nutrient-specific gene expression.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHolger Kramer
SpeciesList scientific name: Schizosaccharomyces pombe OY26; NCBI TaxID: 649908;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-09 23:47:31ID requested
12022-02-24 06:34:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Fission yeast, Schizosaccharomyces pombe, Resource allocation, Proteomics, Transcriptomics, Gene expression
Contact List
Holger Kramer
contact affiliationMRC London Institute of Medical Sciences Proteomics & Metabolomics Facility Hammersmith Hospital Campus Du Cane Road London, W12 0NN United Kingdom
contact emailh.kramer@lms.mrc.ac.uk
lab head
Holger Kramer
contact affiliationMRC London Institute of Medical Sciences
contact emailh.kramer@lms.mrc.ac.uk
dataset submitter
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