PXD027795

PXD027795 is an original dataset announced via ProteomeXchange.

Title	Essential role of Cp190 in physical and regulatory boundary formation in Drosophila
Description	Animal genomes fold into contact domains defined by enhanced internal contact frequencies with debated functions in establishing independent gene regulatory domains. A large fraction of contact domains in mammals are formed by stalling of chromosomal loop-extruding cohesin by CTCF at domain boundaries. 90% of domain boundaries in Drosophila form CTCF-independently, and other proteins were proposed to form chromosomal loops with dual functions of segregating promoters from inappropriate regulatory elements and connecting distal regulatory elements to their correct targets. Here, we genetically ablate the ubiquitous boundary-associated factor Cp190 and assess impacts on genome folding and transcriptional regulation in embryos. Our results reveal that Cp190 is a major factor required for contact domain boundary formation and gene insulation in Drosophila.
HostingRepository	PRIDE
AnnounceDate	2022-05-30
AnnouncementXML	Submission_2022-05-30_02:38:49.178.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrice Waridel
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-06 07:31:15	ID requested
⏵ 1	2022-05-30 02:38:50	announced

Kaushal A, Dorier J, Wang B, Mohana G, Taschner M, Cousin P, Waridel P, Iseli C, Semenova A, Restrepo S, Guex N, Aiden EL, Gambetta MC, Essential role of Cp190 in physical and regulatory boundary formation. Sci Adv, 8(19):eabl8834(2022) [pubmed]

submitter keyword: Drosophila, genome architecture, development

Maria Cristina Gambetta
contact affiliation	Center for Integrative Genomics, University of Lausanne, 1015 Lausanne, Switzerland
contact email	mariacristina.gambetta@unil.ch
lab head
Patrice Waridel
contact affiliation	University of Lausanne
contact email	patrice.waridel@unil.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027795
     1. Label: PRIDE project
     2. Name: Essential role of Cp190 in physical and regulatory boundary formation in Drosophila