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PXD027795 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEssential role of Cp190 in physical and regulatory boundary formation in Drosophila
DescriptionAnimal genomes fold into contact domains defined by enhanced internal contact frequencies with debated functions in establishing independent gene regulatory domains. A large fraction of contact domains in mammals are formed by stalling of chromosomal loop-extruding cohesin by CTCF at domain boundaries. 90% of domain boundaries in Drosophila form CTCF-independently, and other proteins were proposed to form chromosomal loops with dual functions of segregating promoters from inappropriate regulatory elements and connecting distal regulatory elements to their correct targets. Here, we genetically ablate the ubiquitous boundary-associated factor Cp190 and assess impacts on genome folding and transcriptional regulation in embryos. Our results reveal that Cp190 is a major factor required for contact domain boundary formation and gene insulation in Drosophila.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPatrice Waridel
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-06 07:31:15ID requested
12022-05-30 02:38:50announced
Publication List
Kaushal A, Dorier J, Wang B, Mohana G, Taschner M, Cousin P, Waridel P, Iseli C, Semenova A, Restrepo S, Guex N, Aiden EL, Gambetta MC, Essential role of Cp190 in physical and regulatory boundary formation. Sci Adv, 8(19):eabl8834(2022) [pubmed]
Keyword List
submitter keyword: Drosophila, genome architecture, development
Contact List
Maria Cristina Gambetta
contact affiliationCenter for Integrative Genomics, University of Lausanne, 1015 Lausanne, Switzerland
contact emailmariacristina.gambetta@unil.ch
lab head
Patrice Waridel
contact affiliationUniversity of Lausanne
contact emailpatrice.waridel@unil.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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