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PXD027689 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted MRM assay Complement System AMD cohort
DescriptionAge-related macular degeneration (AMD) is a major cause of vision loss among the elderly in the Western world. The complement system has been identified as one of the main AMD disease pathways. We performed a comprehensive expression analysis of 32 complement proteins in plasma samples of 255 AMD patients and 221 control individuals using mass spectrometry-based quantitative multiplex profiling. We detected significant associations of complement protein levels with age, gender and body-mass index (BMI), and potential associations of C-reactive protein, factor H related-2 (FHR-2) and collectin-11 with AMD. In addition, we confirmed previously described associations and identified new associations of AMD variants with complement levels. New associations include increased C4 levels for rs181705462 at the C2/CFB locus, decreased vitronectin (VTN) levels for rs11080055 at the TMEM97/VTN locus, and decreased factor I levels for rs10033900 at the CFI locus. Finally, we detected significant associations between AMD-associated metabolites and complement proteins in plasma. The most significant complement-metabolite associations included increased high density lipoprotein (HDL) subparticle levels with decreased C3, factor H (FH) and VTN levels. The results of our study indicate that demographic factors, genetic variants and circulating metabolites are associated with complement protein components. We suggest that these factors should be taken into account to design personalized treatment approaches and to increase the success of clinical trials targeting the complement system.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterEsther Willems
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentXevo TQ-S
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-08-02 23:04:59ID requested
12021-12-01 13:13:34announced
Publication List
Acar IE, Willems E, Kersten E, Keizer-Garritsen J, Kragt E, Bakker B, Galesloot TE, Hoyng CB, Fauser S, van Gool AJ, Lechanteur YTE, Koertvely E, Nogoceke E, Gloerich J, de Jonge MI, Lorés-Motta L, den Hollander AI. Semi-Quantitative Multiplex Profiling of the Complement System Identifies Associations of Complement Proteins with Genetic Variants and Metabolites in Age-Related Macular Degeneration. Vol. 11, Journal of Personalized Medicine . 2021
Keyword List
submitter keyword: Age-related macular degeneration, AMD, complement system, quantitative multiplex profiling, mass spectrometry, C4, vitronectin, factor I, genetic variants, metabolites, HDL
Contact List
Jolein Gloerich
contact affiliationTranslational Metabolic Laboratory, Department of Laboratory Medicine, Radboud Institute for Molecular Life Sciences, Radboud university medical center, Nijmegen, The Netherlands
contact emailjolein.gloerich@radboudumc.nl
lab head
Esther Willems
contact affiliationSection Pediatric Infectious Diseases, Laboratory of Medical Immunology, Radboud Institute for Molecular Life Sciences, Radboud university medical center, Nijmegen, The Netherlands
contact emailesther.willems1@radboudumc.nl
dataset submitter
Full Dataset Link List
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