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PXD027679

PXD027679 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSample Size-comparable Spectral Library Enhances Data-Independent Acquisition-based Proteome Coverage of Low-input Cells
DescriptionDespite advancement of data-independent acquisition mass spectrometry (DIA-MS) to provide comprehensive and reproducible quantitative proteome profiling, its utility in very low-input samples is limited. For low-input samples at microscale, the proteome composition and overall peptide ion patterns are different from the conventional sample size. Thus, the conventional LC-MS/MS acquisition and widely used large proteome-scale DIA libraries may not be suitable for the microscale sample. In this study, we report a sample size-comparable library-based DIA approach for enhanced proteome coverage of low-input samples at nanoscale to near single-cell levels (i.e. nanogram cells,~5-50 cells). With these spectral libraries made freely available, we envision that such single-shot DIA strategy and the DIA digital map will advance quantitative proteomics applications by enabling improved deep proteome profiling for mass-limited samples.
HostingRepositoryjPOST
AnnounceDate2022-11-07
AnnouncementXMLSubmission_2022-11-06_07:00:04.231.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAsad Ali Siyal
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-31 22:08:03ID requested
12022-11-06 07:00:04announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: microproteomics, nanoproteomics, near single-cell proteomics, data-independent acquisition, mass spectrometry
Contact List
Yu-Ju Chen
lab head
Asad Ali Siyal
contact affiliationAcademia Sinica
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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