Update information. Human infertility has become a medical and social health problem globally. Mice deficient in type 3 adenylyl cyclase (AC3), a key enzyme that synthesizes cyclic adenosine monophosphate (cAMP), develop male infertility but its underlying molecular mechanisms are still unknown. Here, we performed label-free quantitative (LFQ) proteomics study to identify testicular differentially expressed proteins (DEPs) and their respective biological processes.