<<< Full experiment listing

PXD027330

PXD027330 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeubiquitinating enzymes and the proteasome regulate unique sets of ubiquitin substrates.
DescriptionThe ubiquitin-proteasome axis has been extensively explored at a system-wide level, but the impact of deubiquitinating enzymes (DUBs) on the ubiquitinome remains largely unknown. Using UbiSite technology and inhibitors, we have compared the contributions of the proteasome and DUBs on the ubiquitinome. We uncovered large differential dynamic Ub signalling networks with substrates and sites uniquely regulated by DUBs or by the proteasome, highlighting the role of DUBs in degradation-independent ubiquitin signalling. DUBs regulate substrates via nearly 40,000 unique sites. Moreover, we found that ubiquitin conjugated to SUMO2/3 forms a unidirectional proteasomal degradation signal with strikingly rapid kinetics compared to ubiquitin polymers only. We found that PARP1 is hyper ubiquitinated in response to DUB inhibition, increasing its enzymatic activity. Our findings highlight the key regulatory roles of DUBs on ubiquitin dynamics.
HostingRepositoryPRIDE
AnnounceDate2022-05-23
AnnouncementXMLSubmission_2022-05-23_04:50:11.514.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFredrik Trulsson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitinylated lysine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-15 09:28:57ID requested
12022-05-23 04:50:12announced
Publication List
Trulsson F, Akimov V, Robu M, van Overbeek N, Berrocal DAP, Shah RG, Cox J, Shah GM, Blagoev B, Vertegaal ACO, Deubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates. Nat Commun, 13(1):2736(2022) [pubmed]
Keyword List
submitter keyword: Human, U2OS, His10-Ub, Ubiquitin, TAK243, MG132, PR619
Contact List
Alfred Vertegaal
contact affiliationLeiden University Medical Center
contact emailA.C.O.Vertegaal@lumc.nl
lab head
Fredrik Trulsson
contact affiliationLUMC
contact emailc.f.b.trulsson@lumc.nl
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD027330
PRIDE project URI
Repository Record List
[ + ]