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PXD027328 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeubiquitinating enzymes and the proteasome regulate unique sets of ubiquitin substrates.
DescriptionThe ubiquitin-proteasome axis has been extensively explored at a system-wide level, but the impact of deubiquitinating enzymes (DUBs) on the ubiquitinome remains largely unknown. Using UbiSite technology and inhibitors, we have compared the contributions of the proteasome and DUBs on the ubiquitinome. We uncovered large differential dynamic Ub signalling networks with substrates and sites uniquely regulated by DUBs or by the proteasome, highlighting the role of DUBs in degradation-independent ubiquitin signalling. DUBs regulate substrates via nearly 40,000 unique sites. Moreover, we found that ubiquitin conjugated to SUMO2/3 forms a unidirectional proteasomal degradation signal with strikingly rapid kinetics compared to ubiquitin polymers only. We found that PARP1, is hyper ubiquitinated in response to DUB inhibition, increasing its enzymatic activity. Our findings highlight the key regulatory roles of DUBs on ubiquitin dynamics.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFredrik Trulsson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitinylated lysine; phosphorylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-15 09:28:43ID requested
12022-05-23 04:48:30announced
Publication List
Trulsson F, Akimov V, Robu M, van Overbeek N, Berrocal DAP, Shah RG, Cox J, Shah GM, Blagoev B, Vertegaal ACO, Deubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates. Nat Commun, 13(1):2736(2022) [pubmed]
Keyword List
submitter keyword: Human, U2OS, UbiSite, Ubiquitin, TAK243, MG132, PR619
Contact List
Alfred Vertegaal
contact affiliationLeiden Univeristy Medical Center
contact emailA.C.O.Vertegaal@lumc.nl
lab head
Fredrik Trulsson
contact affiliationLUMC
contact emailc.f.b.trulsson@lumc.nl
dataset submitter
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Dataset FTP location
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