PXD027198

PXD027198 is an original dataset announced via ProteomeXchange.

Title	Personalised phosphoproteomics extracts functional signalling
Description	Protein phosphorylation dynamically integrates environmental and cellular information to control virtually all biological processes. Identifying functional phosphorylation amongst the thousands of phosphosites regulated by a perturbation at a global scale is a major challenge. Here, we introduce personalised phosphoproteomics, an experimental and computational framework to link signalling with biological function by exploiting human phenotypic variance. This is achieved by measuring individual subject phosphoproteome responses to interventions with corresponding phenotypes measured in parallel. We employed this method to investigate how exercise potentiates insulin sensitivity in human skeletal muscle. Our approach extracted signalling highly enriched in known functional sites, as well as many previously unknown sites on proteins intimately involved in glucose metabolism. This included a new co-operative relationship between mTOR and AMPK, for which we found a role in metabolic regulation. These results establish personalised phosphoproteomics as a general approach to investigate the signal transduction underlying complex biology.
HostingRepository	PRIDE
AnnounceDate	2021-12-06
AnnouncementXML	Submission_2022-01-19_07:53:11.977.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sean Humphrey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-08 21:36:18	ID requested
1	2021-12-06 09:37:22	announced
⏵ 2	2022-01-19 07:53:12	announced	2022-01-19: Updated publication reference for PubMed record(s): 34857927.

Needham EJ, Hingst JR, Parker BL, Morrison KR, Yang G, Onslev J, Kristensen JM, H, ø, jlund K, Ling NXY, Oakhill JS, Richter EA, Kiens B, Petersen J, Pehm, ø, ller C, James DE, Wojtaszewski JFP, Humphrey SJ, Personalized phosphoproteomics identifies functional signaling. Nat Biotechnol, 40(4):576-584(2022) [pubmed]

submitter keyword: phosphoproteomics, human, skeletal muscle, insulin, exercise

Sean J Humphrey
contact affiliation	The University of Sydney, Australia
contact email	sean.humphrey@sydney.edu.au
lab head
Sean Humphrey
contact affiliation	The University of Sydney
contact email	sean.j.humphrey@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD027198

PRIDE project URI

• PRIDE
  1. PXD027198
     1. Label: PRIDE project
     2. Name: Personalised phosphoproteomics extracts functional signalling