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PXD027198 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePersonalised phosphoproteomics extracts functional signalling
DescriptionProtein phosphorylation dynamically integrates environmental and cellular information to control virtually all biological processes. Identifying functional phosphorylation amongst the thousands of phosphosites regulated by a perturbation at a global scale is a major challenge. Here, we introduce personalised phosphoproteomics, an experimental and computational framework to link signalling with biological function by exploiting human phenotypic variance. This is achieved by measuring individual subject phosphoproteome responses to interventions with corresponding phenotypes measured in parallel. We employed this method to investigate how exercise potentiates insulin sensitivity in human skeletal muscle. Our approach extracted signalling highly enriched in known functional sites, as well as many previously unknown sites on proteins intimately involved in glucose metabolism. This included a new co-operative relationship between mTOR and AMPK, for which we found a role in metabolic regulation. These results establish personalised phosphoproteomics as a general approach to investigate the signal transduction underlying complex biology.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSean Humphrey
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentOrbitrap Exploris 480; Q Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-08 21:36:18ID requested
12021-12-06 09:37:22announced
22022-01-19 07:53:12announced2022-01-19: Updated publication reference for PubMed record(s): 34857927.
Publication List
Needham EJ, Hingst JR, Parker BL, Morrison KR, Yang G, Onslev J, Kristensen JM, H, ø, jlund K, Ling NXY, Oakhill JS, Richter EA, Kiens B, Petersen J, Pehm, ø, ller C, James DE, Wojtaszewski JFP, Humphrey SJ, Personalized phosphoproteomics identifies functional signaling. Nat Biotechnol, 40(4):576-584(2022) [pubmed]
Keyword List
submitter keyword: phosphoproteomics, human, skeletal muscle, insulin, exercise
Contact List
Sean J Humphrey
contact affiliationThe University of Sydney, Australia
contact emailsean.humphrey@sydney.edu.au
lab head
Sean Humphrey
contact affiliationThe University of Sydney
contact emailsean.j.humphrey@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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