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PXD026939 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHighly multiplexed quantitative phosphosite assay for biology and preclinical studies
DescriptionReliable methods to quantify dynamic signaling changes across diverse pathways are needed to better understand the effects of disease and drug treatment in cells and tissues but are presently lacking. Here we present SigPath, a targeted mass spectrometry (MS) assay that measures 284 phosphosites in 200 phosphoproteins of biological interest. SigPath probes a broad swath of signaling biology with high throughput and quantitative precision. We applied the assay to investigate changes in phospho-signaling in drug-treated cancer cell lines, breast cancer preclinical models and human medulloblastoma tumors. In addition to validating previous findings, SigPath detected and quantified a large number of differentially regulated phosphosites newly associated with disease models and human tumors at baseline or with drug perturbation. Our results highlight the potential of SigPath to monitor phosphoproteomic signaling events and to nominate mechanistic hypotheses regarding oncogenesis, response and resistance to therapy.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportsupported by repository but incomplete data and/or metadata
PrimarySubmitterHasmik Keshishian
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListPhospho; Carbamidomethyl; Oxidation; Label:13C(6)15N(1); Label:13C(5)15N(1); Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentTSQ Quantiva
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-27 14:05:46ID requested
12021-10-08 17:02:24announced
Publication List
Keshishian H, McDonald ER, Mundt F, Melanson R, Krug K, Porter DA, Wallace L, Forestier D, Rabasha B, Marlow SE, Jane-Valbuena J, Todres E, Specht H, Robinson ML, Jean Beltran PM, Babur O, Olive ME, Golji J, Kuhn E, Burgess M, MacMullan MA, Rejtar T, Wang K, Mani DR, Satpathy S, Gillette MA, Sellers WR, Carr SA, A highly multiplexed quantitative phosphosite assay for biology and preclinical studies. Mol Syst Biol, 17(9):e10156(2021) [pubmed]
Keyword List
submitter keyword: Proteomics, post-translational modifications, targeted mass spectrometry, multiple reaction monitoring, selected reaction monitoring, breast cancer, medulloblastoma, CPTAC
Contact List
contact affiliationBroad Institute
contact emailscarr@broadinstitute.org
lab head
Hasmik Keshishian
contact affiliationBroad Institute of MIT and Harvard
contact emailhasmik@broadinstitute.org
dataset submitter
Full Dataset Link List
Panorama Public dataset URI