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PXD026748

PXD026748 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome-wide identification of ISG15 sites targeted by SARS-CoV-2 PLpro
DescriptionHere, we applied an ISG15-GlyGly peptidomics approach on HeLa cells producing high levels of ISGylated proteins upon stimulation with IFN-α. After obtaining cellular lysates from wild-type and Isg15-/-cells, the samples were incubated with recombinant wild-type or mutant PLpro to catalyze deISGylation of host cellular proteins. After incubation with PLpro, we continued with trypsin digestion to generate diglycine-modified peptides that were subsequently enriched by immunoprecipitation and identified and quantified by LC-MS/MS. By comparing sites across all four conditions, we uncovered 118 ISGylation sites on 95 proteins as targets of the SARS-CoV-2 protease PLpro.
HostingRepositoryPRIDE
AnnounceDate2021-10-12
AnnouncementXMLSubmission_2021-10-12_07:14:42.370.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDenzel Eggermont
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-16 09:36:03ID requested
12021-10-12 07:14:42announced
Publication List
Dataset with its publication pending
Keyword List
ProteomeXchange project tag: Covid-19
submitter keyword: ISG15, SARS-CoV-2, interferon
Contact List
Francis Impens
contact affiliationVIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
contact emailFrancis.Impens@vib-ugent.be
lab head
Denzel Eggermont
contact affiliationVIB-Ugent Center for Medical Biotechnology
contact emaildenzel.eggermont@vib-ugent.be
dataset submitter
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Dataset FTP location
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