PXD026748 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome-wide identification of ISG15 sites targeted by SARS-CoV-2 PLpro |
Description | Here, we applied an ISG15-GlyGly peptidomics approach on HeLa cells producing high levels of ISGylated proteins upon stimulation with IFN-α. After obtaining cellular lysates from wild-type and Isg15-/-cells, the samples were incubated with recombinant wild-type or mutant PLpro to catalyze deISGylation of host cellular proteins. After incubation with PLpro, we continued with trypsin digestion to generate diglycine-modified peptides that were subsequently enriched by immunoprecipitation and identified and quantified by LC-MS/MS. By comparing sites across all four conditions, we uncovered 118 ISGylation sites on 95 proteins as targets of the SARS-CoV-2 protease PLpro. |
HostingRepository | PRIDE |
AnnounceDate | 2021-10-12 |
AnnouncementXML | Submission_2021-10-12_07:14:42.370.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Denzel Eggermont |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | ubiquitination signature dipeptidyl lysine; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-16 09:36:03 | ID requested | |
⏵ 1 | 2021-10-12 07:14:42 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Covid-19 |
submitter keyword: ISG15, SARS-CoV-2, interferon |
Contact List
Francis Impens |
contact affiliation | VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium |
contact email | Francis.Impens@vib-ugent.be |
lab head | |
Denzel Eggermont |
contact affiliation | VIB-Ugent Center for Medical Biotechnology |
contact email | denzel.eggermont@vib-ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD026748 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026748
- Label: PRIDE project
- Name: Proteome-wide identification of ISG15 sites targeted by SARS-CoV-2 PLpro