PXD026625

PXD026625 is an original dataset announced via ProteomeXchange.

Title	The global Proteome and Ubiquitinome of bacterial and viral co-infected Bronchial Epithelial Cells
Description	Viral infections facilitate bacterial trafficking to the lower respiratory tract resulting in bacterial viral co infections. Bacterial dissemination to the lower respiratory tract is enhanced by influenza A virus induced epithelial cell damage and dysregulation of immune responses. Epithelial cells act as the first line of defense and detect pathogens by a high variety of pattern recognition receptors. The post translational modification ubiquitin is involved in almost every cellular process. Moreover, ubiquitination contributes to the regulation of host immune responses, influenza A virus uncoating and transport within host cells. We applied proteomics with a special focus on ubiquitination to assess the impact of single bacterial and viral as well as bacterial viral co-infections on bronchial epithelial cells. We used Tandem Ubiquitin Binding Entities to enrich polyubiquitinated proteins and assess changes in the ubiquitinome. Infecting 16HBE cells with Streptococcus pyogenes led to an increased abundance of proteins related to mitochondrial translation and energy metabolism in proteome and ubiquitinome. In contrast, influenza A virus infection mainly altered the ubiquitinome. Co-infections had no additional impact on protein abundances or affected pathways. Changes in protein abundance and enriched pathways were assigned to imprints of both infecting pathogens.
HostingRepository	PanoramaPublic
AnnounceDate	2022-06-13
AnnouncementXML	Submission_2022-06-13_13:09:42.012.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Thomas Sura
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Streptococcus pyogenes; NCBI TaxID: 1314; scientific name: Influenza A virus (A/Germany-BY/74/2009(H1N1)); NCBI TaxID: 757400; scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-10 10:18:42	ID requested
⏵ 1	2022-06-13 13:09:42	announced

Sura T, Surabhi S, Maa, ß S, Hammerschmidt S, Siemens N, Becher D, The global proteome and ubiquitinome of bacterial and viral co-infected bronchial epithelial cells. J Proteomics, 250():104387(2022) [pubmed]

submitter keyword: co-infection, influenza A virus, Streptococcus pyogenes, Staphylococcus aureus, 16HBE, proteomics

Dörte Becher
contact affiliation	University of Greifswald, Center for Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics
contact email	doerte.becher@uni-greifswald.de
lab head
Thomas Sura
contact affiliation	University of Greifswald, Center for Functional Genomics of Microbes, Institute of Microbiology, Department of Microbial Proteomics
contact email	thomas.sura@uni-greifswald.de
dataset submitter

Panorama Public dataset URI