PXD026617-1
PXD026617 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted validation of interaction between ErbB4 JM-a isoform and β1 integrin |
Description | The ErbB4 receptor isoforms JM-a and JM-b differ within their extracellular juxtamembrane (eJM) domains. Here we used ErbB4 isoforms as a model to address whether structural variation in the eJM domain of receptor tyrosine kinases (RTK) affects downstream signaling. Analysis of ErbB4 interactome and super-resolution cell imaging indicated association of the JM-a- and JM-b-like receptors with specific signaling complexes at different cell surface compartments. A JM-a-specific sequence motif was discovered, and its presence in the eJM domains of several other human RTKs predicted selective STAT activation indicating a conserved RTK signaling mechanism. The RTKs with the JM-a-like eJM motif activated STAT5a, and those that were JM-b-like (lacking the JM-a-like motif) activated STAT5b and STAT3. TYK2 was found to be necessary for JM-b-stimulated STAT5b activation. The activation of STAT5a by the JM-a-like receptors, in turn, involved specific interaction with oligosaccharides N-linked to cell surface glycoproteins such as β1 integrin. These findings provide evidence for a mechanism linking a ubiquitous extracellular motif in RTKs with selective intracellular STAT signaling. |
HostingRepository | PanoramaPublic |
AnnounceDate | 2022-10-22 |
AnnouncementXML | Submission_2022-10-22_22:33:13.759.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Johannes Merilahti |
SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-06-10 06:53:26 | ID requested | |
⏵ 1 | 2022-10-22 22:33:14 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: ErbB4, JM-a, JM-b, STAT signaling, Receptor tyrosine kinase |
Contact List
Klaus Elenius | |
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contact affiliation | Medicity Research Laboratories and Institute of Biomedicine, Faculty of Medicine, University of Turku, Finland. Turku Bioscience Centre, University of Turku and Åbo Akademi University, Finland |
contact email | klaus.elenius@utu.fi |
lab head | |
Johannes Merilahti | |
contact affiliation | Turku Bioscience Centre, University of Turku and Åbo Akademi University, Finland |
contact email | johannes.merilahti@utu.fi |
dataset submitter |
Full Dataset Link List
Panorama Public dataset URI |