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PXD026546-2

PXD026546 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn extracellular receptor tyrosine kinase motif orchestrating intracellular STAT activation
DescriptionThe ErbB4 receptor isoforms JM-a and JM-b differ within their extracellular juxtamembrane (eJM) domains. Here, ErbB4 isoforms are used as a model to address the effect of structural variation in the eJM domain of receptor tyrosine kinases (RTK) on downstream signaling. A specific JM-a-like sequence motif is discovered, and its presence or absence (in JM-b-like RTKs) in the eJM domains of several RTKs is demonstrated to dictate selective STAT activation. STAT5a activation by RTKs including the JM-a like motif is shown to involve interaction with oligosaccharides of N-glycosylated cell surface proteins such as β1 integrin, whereas STAT5b activation by JM-b is dependent on TYK2. ErbB4 JM-a- and JM-b-like RTKs are shown to associate with specific signaling complexes at different cell surface compartments using analyses of RTK interactomes and super-resolution imaging. These findings provide evidence for a conserved mechanism linking a ubiquitous extracellular motif in RTKs with selective intracellular STAT signaling
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:12:32.964.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJohannes Merilahti
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive HF; LTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-07 06:39:17ID requested
12023-01-30 05:48:29announced
22023-11-14 08:12:35announced2023-11-14: Updated project metadata.
Publication List
Vaparanta K, Jokilammi A, Tamirat M, Merilahti JAM, Salokas K, Varjosalo M, Ivaska J, Johnson MS, Elenius K, An extracellular receptor tyrosine kinase motif orchestrating intracellular STAT activation. Nat Commun, 13(1):6953(2022) [pubmed]
Keyword List
submitter keyword: Receptor tyrosine kinase, JM-a, RTK,STAT signaling, Erbb4, JM-b
Contact List
Klaus Elenius
contact affiliationMedicity Research Laboratories and Institute of Biomedicine, Faculty of Medicine, University of Turku, 20520 Turku, Finland. Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland. Department of Oncology, Turku University Hospital, 20521 Turku, Finland.
contact emailklaus.elenius@utu.fi
lab head
Johannes Merilahti
contact affiliationTurku Bioscience University of Turku
contact emailjohannes.merilahti@utu.fi
dataset submitter
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Dataset FTP location
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