PXD026546 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | An extracellular receptor tyrosine kinase motif orchestrating intracellular STAT activation |
Description | The ErbB4 receptor isoforms JM-a and JM-b differ within their extracellular juxtamembrane (eJM) domains. Here, ErbB4 isoforms are used as a model to address the effect of structural variation in the eJM domain of receptor tyrosine kinases (RTK) on downstream signaling. A specific JM-a-like sequence motif is discovered, and its presence or absence (in JM-b-like RTKs) in the eJM domains of several RTKs is demonstrated to dictate selective STAT activation. STAT5a activation by RTKs including the JM-a like motif is shown to involve interaction with oligosaccharides of N-glycosylated cell surface proteins such as β1 integrin, whereas STAT5b activation by JM-b is dependent on TYK2. ErbB4 JM-a- and JM-b-like RTKs are shown to associate with specific signaling complexes at different cell surface compartments using analyses of RTK interactomes and super-resolution imaging. These findings provide evidence for a conserved mechanism linking a ubiquitous extracellular motif in RTKs with selective intracellular STAT signaling |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:12:32.964.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Johannes Merilahti |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF; LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-07 06:39:17 | ID requested | |
1 | 2023-01-30 05:48:29 | announced | |
⏵ 2 | 2023-11-14 08:12:35 | announced | 2023-11-14: Updated project metadata. |
Publication List
Vaparanta K, Jokilammi A, Tamirat M, Merilahti JAM, Salokas K, Varjosalo M, Ivaska J, Johnson MS, Elenius K, An extracellular receptor tyrosine kinase motif orchestrating intracellular STAT activation. Nat Commun, 13(1):6953(2022) [pubmed] |
Keyword List
submitter keyword: Receptor tyrosine kinase, JM-a, RTK,STAT signaling, Erbb4, JM-b |
Contact List
Klaus Elenius |
contact affiliation | Medicity Research Laboratories and Institute of Biomedicine, Faculty of Medicine, University of Turku, 20520 Turku, Finland. Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland. Department of Oncology, Turku University Hospital, 20521 Turku, Finland. |
contact email | klaus.elenius@utu.fi |
lab head | |
Johannes Merilahti |
contact affiliation | Turku Bioscience University of Turku |
contact email | johannes.merilahti@utu.fi |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026546
- Label: PRIDE project
- Name: An extracellular receptor tyrosine kinase motif orchestrating intracellular STAT activation