PXD026546-2

PXD026546 is an original dataset announced via ProteomeXchange.

Title	An extracellular receptor tyrosine kinase motif orchestrating intracellular STAT activation
Description	The ErbB4 receptor isoforms JM-a and JM-b differ within their extracellular juxtamembrane (eJM) domains. Here, ErbB4 isoforms are used as a model to address the effect of structural variation in the eJM domain of receptor tyrosine kinases (RTK) on downstream signaling. A specific JM-a-like sequence motif is discovered, and its presence or absence (in JM-b-like RTKs) in the eJM domains of several RTKs is demonstrated to dictate selective STAT activation. STAT5a activation by RTKs including the JM-a like motif is shown to involve interaction with oligosaccharides of N-glycosylated cell surface proteins such as β1 integrin, whereas STAT5b activation by JM-b is dependent on TYK2. ErbB4 JM-a- and JM-b-like RTKs are shown to associate with specific signaling complexes at different cell surface compartments using analyses of RTK interactomes and super-resolution imaging. These findings provide evidence for a conserved mechanism linking a ubiquitous extracellular motif in RTKs with selective intracellular STAT signaling
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:12:32.964.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Merilahti
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-07 06:39:17	ID requested
1	2023-01-30 05:48:29	announced
⏵ 2	2023-11-14 08:12:35	announced	2023-11-14: Updated project metadata.

Vaparanta K, Jokilammi A, Tamirat M, Merilahti JAM, Salokas K, Varjosalo M, Ivaska J, Johnson MS, Elenius K, An extracellular receptor tyrosine kinase motif orchestrating intracellular STAT activation. Nat Commun, 13(1):6953(2022) [pubmed]

submitter keyword: Receptor tyrosine kinase, JM-a, RTK,STAT signaling, Erbb4, JM-b

Klaus Elenius
contact affiliation	Medicity Research Laboratories and Institute of Biomedicine, Faculty of Medicine, University of Turku, 20520 Turku, Finland. Turku Bioscience Centre, University of Turku and Åbo Akademi University, 20520 Turku, Finland. Department of Oncology, Turku University Hospital, 20521 Turku, Finland.
contact email	klaus.elenius@utu.fi
lab head
Johannes Merilahti
contact affiliation	Turku Bioscience University of Turku
contact email	johannes.merilahti@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD026546

PRIDE project URI

• PRIDE
  1. PXD026546
     1. Label: PRIDE project
     2. Name: An extracellular receptor tyrosine kinase motif orchestrating intracellular STAT activation