A set of 15 biotinylated SARS-CoV-2 proteins and the total cell lysate from stable isotope labeled HEK 293T cells were prepared. Here we applied SPIDER assay and a SILAC-based mass spectrometry strategy to capture and identify SARS-CoV-2-Human interactome. Biotinylated SARS-CoV-2 proteins was incubated with HEK293T total lysate labeled with heavy stable isotope. As a control, free biotin was incubated with E. coli total lysate labeled with light stable isotope.