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PXD026488

PXD026488 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMonitoring Fe-S cluster occupancy across the E. coli proteome using chemoproteomics
DescriptionIron-sulfur (Fe-S) clusters are ubiquitous metallocofactors involved in redox chemistry, radical generation, and gene regulation. Common methods to monitor Fe-S clusters include spectroscopic analysis of purified proteins, and auto-radiographic visualization of radiolabeled iron distribution in proteomes. Here, we report a chemoproteomic strategy that monitors changes in the reactivity of Fe-S cysteine ligands to inform on Fe-S cluster occupancy. We highlight the utility of this platform in E. coli by: (1) demonstrating global disruptions in Fe-S incorporation in cells cultured under iron-depleted conditions; (2) determining Fe-S client proteins reliant on three scaffold/carrier proteins within the Isc Fe-S biogenesis pathway; and, (3) identifying two previously unannotated Fe-S proteins, TrhP and DppD. In summary, the chemoproteomic strategy described herein is a powerful tool that reports on Fe-S cluster incorporation directly within a native proteome, and enables the interrogation of Fe-S biogenesis pathways, and the identification of previously uncharacterized Fe-S proteins.
HostingRepositoryPRIDE
AnnounceDate2024-05-22
AnnouncementXMLSubmission_2024-05-22_03:07:54.054.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDaniel Bak
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListiodoacetamide derivatized amino-terminal residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-04 06:01:12ID requested
12024-05-22 03:07:54announced
Publication List
Bak DW, Weerapana E, Monitoring Fe-S cluster occupancy across the E. coli proteome using chemoproteomics. Nat Chem Biol, 19(3):356-366(2023) [pubmed]
10.1038/s41589-022-01227-9;
Keyword List
submitter keyword: ReDiMe, Iron-sulfur clusters, isoTOP-ABPP,E. coli
Contact List
Eranthie Weerapana
contact affiliationChemistry Department, Boston College, USA
contact emaileranthie@bc.edu
lab head
Daniel Bak
contact affiliationBoston College
contact emailbakd@bc.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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