PXD026462 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Yield and immunomodulatory effects of pneumococcal membrane vesicles change with bacterial growth phase |
Description | We investigated whether isolation of MVs during either the stationary-phase (24 h) or death-phase (48 h) affected their yield, immunogenicity and cytotoxicity in primary human immune cells. Death-phase vesicles showed higher yield than stationary-phase vesicles. Both vesicle types displayed excellent compatibility with primary human macrophages, and several cell lines, without cytotoxic effect. However, stationary-phase MVs showed slightly higher uptake into primary macrophages. Both vesicle types enhanced the release of inflammatory cytokines tumor necrosis factor and interleukin-6 from primary human macrophages. Proteomic analysis revealed similarities in vesicular immunogenic proteins in both vesicle types, such as pneumolysin, pneumococcal surface protein A, IgA1 protease, but stationary-phase MVs showed significantly lower autolysin levels, than death-phase MVs. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-16 |
AnnouncementXML | Submission_2022-02-16_03:59:28.467.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Tobias Kroniger |
SpeciesList | scientific name: Streptococcus pneumoniae R6; NCBI TaxID: 171101; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-03 06:04:55 | ID requested | |
⏵ 1 | 2022-02-16 03:59:29 | announced | |
Publication List
Mehanny M, Kroniger T, Koch M, Hoppst, ä, dter J, Becher D, Kiemer AK, Lehr CM, Fuhrmann G, Yields and Immunomodulatory Effects of Pneumococcal Membrane Vesicles Differ with the Bacterial Growth Phase. Adv Healthc Mater, 11(5):e2101151(2022) [pubmed] |
Keyword List
submitter keyword: Bacterial membrane vesicles, death-phase, stationary-phase, immunogenicity, proteomics, vaccine, primary macrophages |
Contact List
Dörte Becher |
contact affiliation | Institute of Microbiology, Department of Microbial Proteomics, Center for Functional Genomics of Microbes, University of Greifswald, 17489 Greifswald, Germany |
contact email | dbecher@uni-greifswald.de |
lab head | |
Tobias Kroniger |
contact affiliation | Institute of Microbiology, Department of Microbial Proteomics, Center for Functional Genomics of Microbes, University of Greifswald |
contact email | kronigert@uni-greifswald.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026462
- Label: PRIDE project
- Name: Yield and immunomodulatory effects of pneumococcal membrane vesicles change with bacterial growth phase