PXD026385

PXD026385 is an original dataset announced via ProteomeXchange.

Title	Chromatin transaction map during vertebrate mitotic entry
Description	We have used a combination of chemical genetics, chromatin proteomics and imaging to map the earliest chromatin transactions during vertebrate cell entry into mitosis. Chicken DT40 Cdk1as cells undergo synchronous mitotic entry within 15 minutes following release from a 1NM-PP1-induced arrest in late G2. In addition to changes in chromatin association with nuclear pores and the nuclear envelope, earliest prophase is dominated by changes in the association of ribonucleoproteins with chromatin, particularly in the nucleolus, where pre-rRNA processing factors leave chromatin significantly before RNA polymerase I. Nuclear envelope barrier function is lost early in prophase and cytoplasmic proteins begin to accumulate on the chromatin. As a result, outer kinetochore assembly appears complete by nuclear envelope breakdown (NEBD). Most interphase chromatin proteins remain associated with chromatin until NEBD, after which their levels drop sharply.
HostingRepository	PRIDE
AnnounceDate	2022-01-31
AnnouncementXML	Submission_2022-01-31_03:23:14.399.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georg Kustatscher
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-31 07:30:47	ID requested
⏵ 1	2022-01-31 03:23:15	announced

Samejima I, Spanos C, Samejima K, Rappsilber J, Kustatscher G, Earnshaw WC, Mapping the invisible chromatin transactions of prophase chromosome remodeling. Mol Cell, 82(3):696-708.e4(2022) [pubmed]

submitter keyword: Chicken, Chromatin, Mitosis, SILAC

Juri Rappsilber
contact affiliation	University of Edinburgh
contact email	juri.rappsilber@ed.ac.uk
lab head
Georg Kustatscher
contact affiliation	Wellcome Centre for Cell Biology
contact email	georg.kustatscher@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD026385

PRIDE project URI

• PRIDE
  1. PXD026385
     1. Label: PRIDE project
     2. Name: Chromatin transaction map during vertebrate mitotic entry