PXD026229 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Axonal and somatodendritic proteomes of dopamine neurons in the mouse brain |
Description | Dopamine (DA) neurons modulate neural circuits and behaviors via dopamine release from expansive, long range axonal projections. The elaborate cytoarchitecture of DA neurons is embedded within complex brain tissues, making it difficult to access the DA neuronal proteome using conventional methods. Here, we demonstrate APEX2 proximity labeling within genetically targeted neurons in the mouse brain, enabling subcellular proteomics with cell type-specificity. By combining APEX2 biotinylation with mass spectrometry, we mapped the somatodendritic and axonal proteomes of DA neurons. Our dataset reveals the proteomic architecture underlying axonal transport, dopamine transmission, and axonal metabolism in DA neurons. We find a significant enrichment of proteins encoded by Parkinson’s disease-linked genes in dopaminergic axons, including proteins with previously undescribed axonal localization. Our proteomic datasets comprise a significant resource for axonal and DA neuronal cell biology, while the methodology developed here will enable future studies of other neural cell types. |
HostingRepository | PRIDE |
AnnounceDate | 2021-06-01 |
AnnouncementXML | Submission_2021-06-01_08:41:24.176.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Peter Sims |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-05-24 10:21:17 | ID requested | |
⏵ 1 | 2021-06-01 08:41:24 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: proteomics, APEX2, dopamine neurons, axon |
Contact List
Peter Sims |
contact affiliation | Columbia University Irving Medical Center |
contact email | pas2182@columbia.edu |
lab head | |
Peter Sims |
contact affiliation | Columbia University |
contact email | pas2182@columbia.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD026229 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026229
- Label: PRIDE project
- Name: Axonal and somatodendritic proteomes of dopamine neurons in the mouse brain