PXD025982

PXD025982 is an original dataset announced via ProteomeXchange.

Title	RapiGest Precipitation Depends on Peptide Concentration
Description	The mass spectrometry-compatible surfactant RapiGest promotes the enzymatic digestion of proteins by facilitating their unfolding while retaining enzymatic activity. RapiGest consists of a hydrophilic head and a hydrophobic tail, which can be separated by acid hydrolysis. This allows for removal of RapiGest prior to mass spectrometric analysis via precipitation and solid phase extraction. During in-solution digestion experiments with RapiGest, we noticed a high variability in the formation of precipitates after acid hydrolysis, implying that RapiGest precipitation is sample-dependent. We show that RapiGest hydrolyzes efficiently under acidic conditions and that differences in precipitation are solely due to protein/peptide concentration. Furthermore, we demonstrate that RapiGest precipitation can be triggered by the addition of intact proteins, providing a strategy for its efficient removal from highly diluted samples.
HostingRepository	PRIDE
AnnounceDate	2021-09-07
AnnouncementXML	Submission_2021-09-07_04:47:17.633.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Hardt
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-12 08:47:21	ID requested
⏵ 1	2021-09-07 04:47:18	announced

Mosen PR, Hardt R, Winter D, RapiGest precipitation depends on peptide concentration. Proteomics, 21(20):e2100129(2021) [pubmed]

submitter keyword: LC-MS, RapiGest, in-solution digestion, precipitation, detergent

Dominic Winter
contact affiliation	Institut fuer Biochemie und Molekularbiologie (IBMB,) Med. Fak. Universtitaet Bonn, Nussallee 11, 53115 Bonn, Germany
contact email	dominic.winter@uni-bonn.de
lab head
Robert Hardt
contact affiliation	IBMB - University of Bonn
contact email	rhardt@uni-bonn.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025982
     1. Label: PRIDE project
     2. Name: RapiGest Precipitation Depends on Peptide Concentration