Update publication information. Proteomics has been used extensively in life mechanism studies, drug target and clinical disease diagnosis, and treatment research.Several clinical surgery tissue samples inevitably get contaminated with blood during cryopreservation. This might detrimentally affect the results obtained by the commonly used proteomics DDA data acquisition strategy.For disease research, each clinical sample is precious and sample collection usually takes months or years. Removal of unqualified samples before the experiment is necessary but regrettable. The present findings demonstrate that for clinical tissue samples, compared with DDA strategy, the data derived using DIA is more complete and more potential biomarkers can be identified. DIA strategies can effectively overcome the interference from blood contaminants, allowing greater utilization of existing frozen samples. DIA has excellent analytical capability and application prospects.