PXD025838

PXD025838 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of induced pluripotent stem cell-derived kidney organoids
Description	Kidney organoids were generated from a control iPSC line using a previously published protocol (https://doi.org/10.1038/nprot.2016.098). Organoids were collected at three timepoints during the protocol (day 14, 18 and 25) and prepared for proteomic analyses. The focus of the study was to define the proteomic composition of kidney organoids during differentiation with a particular emphasis on the extracellular matrix and its comparison to in vivo systems. Following a ample fractionation and matrix enrichment strategy, samples were prepared for high resolution label-free tandem mass spectrometry to define the proteomic composition of human kidney organoids.
HostingRepository	PRIDE
AnnounceDate	2021-10-13
AnnouncementXML	Submission_2021-10-12_23:55:23.105.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louise Hopkinson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-05-06 07:18:32	ID requested
⏵ 1	2021-10-12 23:55:23	announced

Dataset with its publication pending

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: Kidney, Kidney organoid, extracellular matrix, enrichment, mass spectrometry, basement membrane

Rachel Lennon
contact affiliation	Wellcome Centre for Cell-matrix biology, University of Manchester, UK.
contact email	rachel.lennon@manchester.ac.uk
lab head
Louise Hopkinson
contact affiliation	University of Manchester
contact email	louise.hopkinson@postgrad.manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD025838

PRIDE project URI

• PRIDE
  1. PXD025838
     1. Label: PRIDE project
     2. Name: Proteomic analysis of induced pluripotent stem cell-derived kidney organoids