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PXD025474 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIn vivo Analysis Reveals that ATP-hydrolysis Couples Remodeling to SWI/SNF Release from Chromatin.
DescriptionATP-dependent chromatin remodelers control the accessibility of genomic DNA through nucleosome mobilization. However, the dynamics of genome exploration by remodelers, and the role of ATP hydrolysis in this process remain unclear. We used live-cell imaging of Drosophila polytene nuclei to monitor Brahma (BRM) remodeler interactions with its chromosomal targets. In parallel, we measured local chromatin condensation and its effect on BRM association. Surprisingly, only a small portion of BRM is bound to chromatin at any given time. BRM binds decondensed chromatin but is excluded from condensed chromatin, limiting its genomic search space. BRM-chromatin interactions are highly dynamic, whereas histone-exchange is limited and much slower. Intriguingly, loss of ATP hydrolysis enhanced chromatin retention and clustering of BRM, which was associated with reduced histone turnover. Thus, ATP hydrolysis couples nucleosome remodeling to remodeler release, driving a continuous transient probing of the genome.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJeroen Demmers
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListacetylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-19 07:49:46ID requested
12022-01-20 01:00:24announced
Publication List
Tilly BC, Chalkley GE, van der Knaap JA, Moshkin YM, Kan TW, Dekkers DH, Demmers JA, Verrijzer CP, In vivo analysis reveals that ATP-hydrolysis couples remodeling to SWI/SNF release from chromatin. Elife, 10():(2021) [pubmed]
Keyword List
submitter keyword: ATP-dependent chromatin remodeling, SWI/SNF, Brahma, live-cell imaging, Ecdysone receptor, Polytene chromosomes
Contact List
Jeroen Demmers
contact affiliationProteomics Center, Erasmus University Medical Center Rotterdam
contact emailj.demmers@erasmusmc.nl
lab head
Jeroen Demmers
contact affiliationProteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands
contact emailj.demmers@erasmusmc.nl
dataset submitter
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Dataset FTP location
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