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PXD025378 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEvidence for a Nuclear Role for Drosophila Dlg as a Regulator of the NURF Complex
DescriptionScrib, Dlg, and Lgl are basolateral regulators of epithelial polarity and tumor suppressors whose molecular mechanisms of action remain unclear. We used proximity biotinylation to identify proteins localized near Dlg in the Drosophila wing imaginal disc epithelium. In addition to expected membrane- and cytoskeleton-associated protein classes, nuclear proteins were prevalent in the resulting mass spectrometry data set, including all four members of the NURF chromatin remodeling complex. Subcellular fractionation demonstrated a nuclear pool of Dlg and proximity ligation confirmed its position near the NURF complex. Genetic analysis showed that NURF activity is also required for the overgrowth of dlg tumors, and this growth suppression correlated with a reduction in Hippo pathway gene expression. Together, these data suggest a nuclear role for Dlg in regulating chromatin and transcription through a more direct mechanism than previously thought.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrett Phinney
SpeciesList scientific name: Drosophila melanogaster; common name: fruit fly; NCBI TaxID: 7227;
ModificationListCarbamidomethyl; Oxidation; TMT6plex
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-04-13 17:06:38ID requested
12021-10-25 12:48:37announced
Publication List
Sharp KA, Khoury MJ, Wirtz-Peitz F, Bilder D, Dlg as a regulator of the NURF complex. Mol Biol Cell, 32(21):ar23(2021) [pubmed]
Keyword List
submitter keyword: NURF Complex
Contact List
David Bilder
contact affiliationDepartment of Molecular and Cell Biology, University of California-Berkeley, Berkeley CA
contact emailbilder@berkeley.edu
lab head
Brett Phinney
contact affiliationUC Davis
contact emailbrettsp@ucdavis.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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