Update publication information. Mitochondria are essential organelles that provide energy for mammalian cells, with multiple functions such as signal transduction, cellular differentiation, and regulation of apoptosis. Compared with the mitochondria in somatic cells, oocyte mitochondria have an additional layer of importance since they are required for germ cell maturation, dysfunctions of which can lead to severe inherited disorders. Thus, a systematic proteomic profile of oocyte mitochondria is urgently needed for basic and clinical research, but has been hindered by the rarity of oocyte samples and technical challenges associated with capturing mitochondrial proteins from live oocytes. Here, in this work, using proximity labeling proteomics, we established a mitochondria-specific ascorbate peroxidase (APEX2) reaction in live GV staged oocytes of mouse and and explore oocyte mitochondrial proteomics.