Update publication information. Plant fungal pathogens secrete numerous proteins into the apoplast at the plant-fungus contact sites to facilitate colonization. Only a limited number of secreted proteins have been functionally characterized in Magnaporthe oryzae, the causal agent of blast disease in rice (Oryza sativa). Intriguingly, the ∆alg3 mutant of M. oryzae, which -1, 3-mannosyltransferase ALG3, shows strong defects in fungal cell wall integrity and virulence, indicating a potential effect on the secretion of multiple proteins. Therefore, in this study, we used a proteomics approach, comparing wild type and ∆alg3 mutants, to identify 51 proteins that require ALG3 for proper secretion. Elucidation of the comparative secretome of M. oryzae improves our understanding of the proteins that require ALG3 for secretion, and of their function in fungal virulence and cell wall integrity.