Lung cancer remains the leading cause of mortality from malignant tumors, non-small cell lung cancer (NSCLC) accounts for the majority of lung cancer cases, and individualized diagnosis and treatment in traditional Chinese Medicine (TCM) is an effective trend. In this study, a proteomics research with DIA mode and a non-targeted lipidomics research were developed in NSCLC patients with Qi deficiency and Yin deficiency (QDYD) and Qi deficiency of lung-spleen (QDLS) syndromes.