PXD024332 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteome-wide effects of single gene perturbations in a eukaryotic cell |
Description | Protein expression levels are controlled at the transcriptional, translational and post-translational level and their regulatory principles are starting to emerge. While transcriptional outcomes, which are commonly also used as a proxy for protein abundance, have been investigated on a larger scale, the study of translational output requires large-scale proteomics data. However, data for proteome alterations by systematic assessment of knockouts genome-wide is not available yet. We here determined the individual proteome changes for 3,308 non-essential genes in the yeast S. pombe. We observed that genes with high proteome remodeling are predominantly involved in gene expression regulation, in particular acting as translational regulators. Focusing on those knockout strains with a large number of altered proteins, we performed paired transcriptome/proteome measurements to uncover translational regulators and features of translational regulation. Furthermore, by similarity clustering of these proteome changes, we infer gene functionality that can be extended to other species such as human or baker’s yeast. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-22 |
AnnouncementXML | Submission_2022-09-22_01:42:10.033.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | F Butter |
SpeciesList | scientific name: Schizosaccharomyces pombe OY26; NCBI TaxID: 649908; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-02-23 23:33:48 | ID requested | |
⏵ 1 | 2022-09-22 01:42:10 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LC-MS/MS |
knockout strain |
Contact List
Falk Butter |
contact affiliation | IMB, Ackermannweg 4, 55128 Mainz |
contact email | f.butter@imb-mainz.de |
lab head | |
F Butter |
contact affiliation | Quantitative Proteomics Institute of Molecular Biology (IMB) |
contact email | f.butter@imb-mainz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024332
- Label: PRIDE project
- Name: Proteome-wide effects of single gene perturbations in a eukaryotic cell