Different studies in the last decade have shown that cells of the innate immune system, such as monocytes and macrophages, can present non-specific memory and enhanced responsiveness to secondary stimulation after challenge with several stimuli, such as beta-glucan or BCG, what has been termed trained immunity. These changes are posible thanks to deep metabolic and epigenetic reprogramming in these cells, which allows the aforementioned enhanced responsiveness after subsequent stimulation. In this project we intend to study the changes in proteome upon induction of trained immunity or tolerance in monocytes.