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PXD024038 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative Proteomics Identifies Protein Tyrosine Phosphatase 1B as Modulator of B Cell Antigen Receptor Signaling
DescriptionB cell antigen receptor (BCR) signaling is initiated by protein kinases and limited by counteracting phosphatases that currently are less well studied in their regulation of BCR signaling. We here used the B cell line Ramos to identify and quantify human B cell signaling components. Specifically, a protein tyrosine phosphatase profiling revealed a high expression of the protein tyrosine phosphatase 1B (PTP1B) in Ramos and human naive B cells. The loss of PTP1B leads to increased B cell activation. Through substrate trapping in combination with quantitative mass spectrometry, we identified 22 putative substrates or interactors of PTP1B. We validated Igalpha, CD22, PLCgamma1/2, CBL, BCAP and APLP2 as specific substrates of PTP1B in Ramos B cells. The tyrosine kinase BTK and the two adaptor proteins GRB2 and VAV1 were identified as direct binding partners and potential substrates of PTP1B. We showed that PTP1B dephosphorylates the inhibitory receptor protein CD22 at phosphotyrosine 807. We conclude that PTP1B negatively modulates BCR signaling by dephosphorylating distinct phosphotyrosines in B cell specific receptor proteins and various downstream signaling components.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJennifer Schwarz
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-02-08 00:06:57ID requested
12022-02-16 13:16:49announced
Publication List
Schwarz JJ, Grundmann L, Kokot T, Kl, ä, sener K, Fotteler S, Medgyesi D, K, ö, hn M, Reth M, Warscheid B, Quantitative proteomics identifies PTP1B as modulator of B cell antigen receptor signaling. Life Sci Alliance, 4(11):(2021) [pubmed]
Keyword List
submitter keyword: Ramos B cells, BCR signaling, Whole proteome analysis, PTP1B knockout, CRISPR, SILAC
Contact List
Bettina Warscheid
contact affiliationBiochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany
contact emailbettina.warscheid@biologie.uni-freiburg.de
lab head
Jennifer Schwarz
contact affiliationUniversity of Freiburg
contact emailjennifer.schwarz@sgbm.uni-freiburg.de
dataset submitter
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Dataset FTP location
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