PXD024038

PXD024038 is an original dataset announced via ProteomeXchange.

Title	Quantitative Proteomics Identifies Protein Tyrosine Phosphatase 1B as Modulator of B Cell Antigen Receptor Signaling
Description	B cell antigen receptor (BCR) signaling is initiated by protein kinases and limited by counteracting phosphatases that currently are less well studied in their regulation of BCR signaling. We here used the B cell line Ramos to identify and quantify human B cell signaling components. Specifically, a protein tyrosine phosphatase profiling revealed a high expression of the protein tyrosine phosphatase 1B (PTP1B) in Ramos and human naive B cells. The loss of PTP1B leads to increased B cell activation. Through substrate trapping in combination with quantitative mass spectrometry, we identified 22 putative substrates or interactors of PTP1B. We validated Igalpha, CD22, PLCgamma1/2, CBL, BCAP and APLP2 as specific substrates of PTP1B in Ramos B cells. The tyrosine kinase BTK and the two adaptor proteins GRB2 and VAV1 were identified as direct binding partners and potential substrates of PTP1B. We showed that PTP1B dephosphorylates the inhibitory receptor protein CD22 at phosphotyrosine 807. We conclude that PTP1B negatively modulates BCR signaling by dephosphorylating distinct phosphotyrosines in B cell specific receptor proteins and various downstream signaling components.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_13:16:48.937.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Schwarz
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-02-08 00:06:57	ID requested
⏵ 1	2022-02-16 13:16:49	announced

Schwarz JJ, Grundmann L, Kokot T, Kl, ä, sener K, Fotteler S, Medgyesi D, K, ö, hn M, Reth M, Warscheid B, Quantitative proteomics identifies PTP1B as modulator of B cell antigen receptor signaling. Life Sci Alliance, 4(11):(2021) [pubmed]

submitter keyword: Ramos B cells, BCR signaling, Whole proteome analysis, PTP1B knockout, CRISPR, SILAC

Bettina Warscheid
contact affiliation	Biochemistry and Functional Proteomics, Institute of Biology II, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany
contact email	bettina.warscheid@biologie.uni-freiburg.de
lab head
Jennifer Schwarz
contact affiliation	University of Freiburg
contact email	jennifer.schwarz@sgbm.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024038
     1. Label: PRIDE project
     2. Name: Quantitative Proteomics Identifies Protein Tyrosine Phosphatase 1B as Modulator of B Cell Antigen Receptor Signaling