PXD023761

PXD023761 is an original dataset announced via ProteomeXchange.

Title	Quantification of organelle membrane contact site protein abundances during infection with human viruses
Description	Viruses rely on organelles to invade, replicate, and spread between host cells. Likewise, organelles underlie the host's ability to sense and respond to pathogen invasion. To subvert cellular functions and turn them to the benefit of virus production and spread, viruses remodel organelle structure and function during infection. We predicted that membrane contact sites (MCSs) underlie virus-driven organelle remodeling events. MCSs use protein interactions to bridge organelle membranes for the direct transfer of biomolecules, coordinating fundamental organelle dynamics. The extent of contact and potential functions of MCSs are dictated by the abundance of MCS-specific proteins at organelle interfaces. Here, we design a parallel reaction monitoring (PRM) targeted MS assay to quantify MCS protein abundances across time and space. Our assay encompasses nearly all MCS functions and all major cellular organelles (ER, mitochondria, peroxisome, endosomes, lysosomes, autophagosomes, lipid droplets, plasma membrane, Golgi), with 2-5 signature peptides per protein and internal controls. Utilizing MCS-PRM, we uncover temporal- and organelle-specific regulation of MCSs during the infectious cycles of critical human viruses: the beta-herpesvirus human cytomegalovirus (HCMV, also known as HHV-5), herpes simplex virus type 1 (HSV-1), the orthomyxovirus influenza A (Infl. A PR8), and the beta-coronavirus HCoV-OC43.
HostingRepository	PanoramaPublic
AnnounceDate	2022-07-08
AnnouncementXML	Submission_2022-07-08_12:05:42.904.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katelyn Cook
SpeciesList	scientific name: Human alphaherpesvirus 1; NCBI TaxID: 10298; scientific name: Influenza A virus (A/Puerto Rico/8/1934(H1N1)); NCBI TaxID: 211044; scientific name: Human betaherpesvirus 5; NCBI TaxID: 10359; scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Human coronavirus OC43; NCBI TaxID: 31631;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-22 11:01:49	ID requested
⏵ 1	2022-07-08 12:05:43	announced

Dataset with its publication pending

submitter keyword: organelle, membrane contact site, organelle contacts, virus, HCMV, HSV-1, influenza A, HCoV-OC43, PRM, MCS-PRM

Ileana Cristea
contact affiliation	Department of Molecular Biology, Princeton University
contact email	icristea@princeton.edu
lab head
Katelyn Cook
contact affiliation	Department of Molecular Biology, Princeton University
contact email	katelync@princeton.edu
dataset submitter

Panorama Public dataset URI