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PXD023761 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantification of organelle membrane contact site protein abundances during infection with human viruses
DescriptionViruses rely on organelles to invade, replicate, and spread between host cells. Likewise, organelles underlie the host's ability to sense and respond to pathogen invasion. To subvert cellular functions and turn them to the benefit of virus production and spread, viruses remodel organelle structure and function during infection. We predicted that membrane contact sites (MCSs) underlie virus-driven organelle remodeling events. MCSs use protein interactions to bridge organelle membranes for the direct transfer of biomolecules, coordinating fundamental organelle dynamics. The extent of contact and potential functions of MCSs are dictated by the abundance of MCS-specific proteins at organelle interfaces. Here, we design a parallel reaction monitoring (PRM) targeted MS assay to quantify MCS protein abundances across time and space. Our assay encompasses nearly all MCS functions and all major cellular organelles (ER, mitochondria, peroxisome, endosomes, lysosomes, autophagosomes, lipid droplets, plasma membrane, Golgi), with 2-5 signature peptides per protein and internal controls. Utilizing MCS-PRM, we uncover temporal- and organelle-specific regulation of MCSs during the infectious cycles of critical human viruses: the beta-herpesvirus human cytomegalovirus (HCMV, also known as HHV-5), herpes simplex virus type 1 (HSV-1), the orthomyxovirus influenza A (Infl. A PR8), and the beta-coronavirus HCoV-OC43.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterKatelyn Cook
SpeciesList scientific name: Human alphaherpesvirus 1; NCBI TaxID: 10298; scientific name: Influenza A virus (A/Puerto Rico/8/1934(H1N1)); NCBI TaxID: 211044; scientific name: Human betaherpesvirus 5; NCBI TaxID: 10359; scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Human coronavirus OC43; NCBI TaxID: 31631;
ModificationListLabel:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-22 11:01:49ID requested
12022-07-08 12:05:43announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: organelle, membrane contact site, organelle contacts, virus, HCMV, HSV-1, influenza A, HCoV-OC43, PRM, MCS-PRM
Contact List
Ileana Cristea
contact affiliationDepartment of Molecular Biology, Princeton University
contact emailicristea@princeton.edu
lab head
Katelyn Cook
contact affiliationDepartment of Molecular Biology, Princeton University
contact emailkatelync@princeton.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI