PXD023668

PXD023668 is an original dataset announced via ProteomeXchange.

Title	Yeast response to protein misfolding induced by azetidine-2-carboxylic acid (AZC)
Description	To identify proteins that interact with the yeast Tsa1 peroxiredoxin, we utilized an N-terminal Myc-tagged version of Tsa1 with a mutation in its resolving cysteineresidue (Tsa1-C171S) to trap and detect redox-dependent interactions. Yeast cells were left untreated, or treated with AZC or hydrogen peroxide prior to immunoprecipitation and mass spectrometry used to identify co-immunoprecipitating proteins.
HostingRepository	PRIDE
AnnounceDate	2021-09-10
AnnouncementXML	Submission_2021-09-09_23:48:16.123.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chris Grant
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-19 06:48:08	ID requested
⏵ 1	2021-09-09 23:48:16	announced

Kritsiligkou P, Nowicki-Osuch K, Carter Z, Kershaw CJ, Creamer DR, Weids AJ, Grant CM, Tolerance to nascent protein misfolding stress requires fine-tuning of the cAMP/PKA pathway. J Biol Chem, 296():100690(2021) [pubmed]

submitter keyword: Yeast, protein misfolding, peroxiredoxin

Chris Grant
contact affiliation	University of Manchester
contact email	chris.grant@manchester.ac.uk
lab head
Chris Grant
contact affiliation	University of Manchester
contact email	chris.grant@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD023668

PRIDE project URI

• PRIDE
  1. PXD023668
     1. Label: PRIDE project
     2. Name: Yeast response to protein misfolding induced by azetidine-2-carboxylic acid (AZC)