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PXD023570 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHeart extracellular vesicle proteome
DescriptionCardiac function is dynamically regulated by intercellular signalling. As an important intercellular signalling mediator, this study focused on isolating and defining membrane bound signalling packages termed extracellular vesicles (EVs) directly from heart. Here, we utilised a gentle, enzymatic perfusion to extract EVs from whole cardiac tissues (cEVs) for biophysical and proteomic characterisation; cEVs (100-300 nm) are Cd63+, Tsg101+, Pdcd6ip/Alix+ (EV markers) and contain proteins associated with cardiomyocyte (Actn1, Myh6), cardiac progenitor (Cd34, Abcg2), smooth muscle (Cald1, Tagln), endothelial (Vwf), and fibroblast (Ckap4) origins. Proteomic profiling of cEVs revealed 1721 proteins, which bioinformatics revealed hold an enrichment in metabolic functions of glycolysis, fatty acid oxidation, and antioxidant action, presumably to meet the high energetic demands and balance resulting oxidative stress in cardiac tissue.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterdavid greening
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-01-13 02:28:37ID requested
12021-04-25 21:27:13announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Heart, Extracellular Vesicles, Signaling, Proteomics
Contact List
David Greening
contact affiliationBaker Heart and Diabetes Institute
contact emaildavid.greening@baker.edu.au
lab head
david greening
contact affiliationBaker Heart and Diabetes Inst.
contact emaildavid.greening@baker.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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