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PXD023325

PXD023325 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStreamlined Single-cell Proteomics by an Integrated Microfluidic Chip and Data-independent Acquisition Mass Spectrometry
DescriptionSingle-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific functional networks underlying biological processes. Here, we present a streamlined workflow combining microfluidic chips for all-in-one proteomic sample preparation and data-independent acquisition (DIA) mass spectrometry (MS) for proteomics analysis down to the single-cell level. The proteomics chips enable multiplexed and automated cell isolation/counting/imaging and sample processing in a single device. Combining chip-based sample handling with DIA-MS using project-specific mass spectral libraries, we profile on average ~1,500 protein groups across 20 single mammalian cells. Applying the chip-DIA workflow to profile the proteomes of adherent and non-adherent malignant cells, we cover a dynamic range of 5 orders of magnitude with good reproducibility and <16% missing values between runs. Taken together, the chip-DIA workflow offers all-in-one cell characterization, analytical sensitivity and robustness, and the option to add additional functionalities in the future, thus providing a basis for advanced single-cell proteomics applications.
HostingRepositoryjPOST
AnnounceDate2021-11-12
AnnouncementXMLSubmission_2022-09-18_03:25:14.656.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAsad Ali Siyal
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-28 20:22:21ID requested
12021-11-12 01:53:51announced
22022-09-18 03:25:15announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: single-cell proteomics, microproteomics, microfluidic chip, data-independent acquisition, mass spectrometry
Contact List
Hsiung-Lin Tu and Yu-Ju Chen
lab head
Asad Ali Siyal
contact affiliationAcademia Sinica
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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