PXD023291 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SWATH-MS and MRM: Quantification of Ras-related proteins in HIV-1 Infected and Methamphetamine-Exposed Human Monocyte-Derived Macrophages (hMDM) |
Description | Macrophages are involved in the immunological response to inflammatory and infectious agents, including Human Immunodeficiency Virus type 1 (HIV-1) infection. They are a primary targets of the virus and facilitate transfer across the blood brain barrier into the central nervous system. In the presence of toxic substances, such as methamphetamine (Meth), the protective functions of macrophages are further impaired. It has been shown that both HIV-1 infection and Meth induce changes in the proteome of macrophages. Therefore, in the presented study we employed a nano-liquid chromatography (nanoLC)-SWATH mass spectrometry (MS) approach to detect and quantify changes on proteome level that are induced in human monocyte derived macrophages (hMDM) that were exposed to Meth before or after HIV infection. As we focused in this study on actin and a cluster of Ras-related proteins (Rab), we selected the following proteins: actin (UniProt P60709), Ras-related protein Rab-1A (UniProt P62820), Ras-related protein Rab-2A (UniProt P61019), Ras-related protein Rab-4B (UniProt P61018), Ras-related protein Rab-7A (UniProt P51149), Ras-related protein Rab-9A (UniProt P51151), Ras-related protein Rab-11 (UniProt P62491), Ras-related protein Rab-14 (UniProt P61106), Ras-related protein Rab-22A (UniProt Q9UL26), Ras-related protein Rab-24 (UniProt Q969Q5), Ras-related protein Rab-37 (UniProt Q96AX2) for further validation, which we performed using liquid chromatography-multiple reaction monitoring (LC-MRM) MS technique. The MRM data from this study, along with LC-MRM analysis and data processing parameters, were separately described and deposited in PASSEL repository with accession number PASS01591. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-19 |
AnnouncementXML | Submission_2022-05-19_13:55:42.467.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Katarzyna Macur |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-23 05:13:40 | ID requested | |
⏵ 1 | 2022-05-19 13:55:43 | announced | |
Publication List
Grabowska K, Macur K, Zieschang S, Zaman L, Haverland N, Schissel A, Morsey B, Fox HS, Ciborowski P, HIV-1 and methamphetamine alter galectins -1, -3, and -9 in human monocyte-derived macrophages. J Neurovirol, 28(1):99-112(2022) [pubmed] |
Keyword List
submitter keyword: HIV, macrophages, Ras-related proteis (Rab), actin, methamphetamine, SWATH |
Contact List
Prof. Pawel Ciborowski |
contact affiliation | Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center, 985800 University of Nebraska Medical Center, Omaha, NE 68198-5800, USA |
contact email | pciborowski@unmc.edu |
lab head | |
Katarzyna Macur |
contact affiliation | University of Nebraska Medical Center |
contact email | kasia.macur@unmc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023291
- Label: PRIDE project
- Name: SWATH-MS and MRM: Quantification of Ras-related proteins in HIV-1 Infected and Methamphetamine-Exposed Human Monocyte-Derived Macrophages (hMDM)