To explore the adaptive mechanisms of soybean in lipid signalling and remodelling of metabolism following salt stress, lipid profiling was performed on leaves of seedlings from cultivar C08 which is a salt-sensitive variety. In our previous study, C08 seedlings showed leaf drooping at 0.5 h under high salt stress followed by recovery. To study if this phenomenon could be closely linked to the dynamic changes in membrane lipid composition, integrated proteomic and lipidomic analyses were conducted. To address protein function in response to salt, label-free quantitative (LFQ) proteomic analysis on salt-treated soybean seedling leaves was conducted. Our study identified putative enzymes or protein components involved in lipid regulation and provides a comprehensive understanding of lipid remodelling following salt treatment in soybean.